Autophagic Flux Measurement
To monitor autophagic flux following drug treatment.
Features of the assay
- Quantitative autophagy detection by fluorescence microscopy.
Cells were treated to accumulate autophagic vescicles. Nuclei were highlighted in blue and autophagic vescicles in green. Left image: untreated cells. Center image: treated cells. The peak of green fluorescence intensity in each cell, proportional to the presence of autophagic vescicles, was quantified by digital imaging. 500 cells were quantified for each condition and Mann-Whitney U test was performed to assess statistical differences.