A new DNA stain for long-term live-cell microscopy

In order to track individual cells in live-cell imaging, fluorescent DNA stains can be used as intracellular reference markers. Hoechst 33342 would be the best option to monitor cell proliferation in live-cell imaging since it is highly selective for DNA, fluorogenic in wash-free imaging and applicable in different cell types. However, Hoechst 33342 shows toxicity to live cells in long-term exposure because of the phototoxic effect of blue light required for its excitation.

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