Cytotoxicity Assays

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Cell Viability                

In vitro drug efficacy.                                             

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Kinetics of Cytotoxicity

Kinetics of drug potency and efficacy on 2D cultures.

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Kinetics of 3D Growth

Kinetics of drug efficacy on 3D tumor spheroids.

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Studies of the Mechanism of Action (MOA)

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Cell Cycle Profiling

Cell cycle distribution with live-cell imaging.         

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Autophagy

Measure compound-mediated autophagy.           

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Apoptosis

Measure compound-mediated activation of apoptosis.

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Senescence

Measure compound-mediated cellular senescence.

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Multi-Drug Resistance

Measure inhibition of ABC transporters.                        

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HDAC Activity             

Determine potency of histone deacetylase inhibitors.            

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ROS Levels

Determine anti-oxidant activity of tested compounds.

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Gene Expr. Profiling

Identify drug-induced cancer-related genes.            

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Proteome Profiling

Identify compound-induced cancer-related proteins.       

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HPBMC Drug Delivery

Nanoparticle-based drug delivery in HPBMC.           

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Spheroid Drug Delivery

Nanoparticle-based drug delivery in tumor spheroids.

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