Kinetics of Tumor Spheroid Growth and Survival

To determine kinetics of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

• Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
• Spheroid growth and death calculation.
• Available for high-throughput screenings.
• Accurate.
• ​Distinction between cytostatic and cytotoxic effects.

Figure 1. Tumor spheroids treated with various compounds.

Detailed description

In standard 2D monolayer critical factors of tumors, such as cell-cell and cell-matrix interactions, do not form. In contrast, 3D culture systems offer the advantage of a more tissue-like complexity and heterogeneity, cellular polarity and interactions. Volume and dead cells inside the spheroids, highlighted in green in the image on the right, are monitored over time through live-imaging.
​

BACK TO OUR LIST OF SERVICES >>

READ ABOUT OUR CONTRACT RESEARCH >>

​READ ABOUT CELL LINES >>
​
​READ MORE ABOUT THE PHYSIOLOGICAL RELEVANCE OF 3D MODELS >>