Kinetics of Tumor Spheroid Growth and Survival

To determine kinetics of compound efficacy on 3D tumor spheroids based on live-cell imaging.
Features of the assay
  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Spheroid growth and death calculation.
  • Available for high-throughput screenings.
  • Accurate.
  • ​Distinction between cytostatic and cytotoxic effects.
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Tumor spheroids treated with various compounds.
Detailed description
In standard 2D monolayer critical factors of tumors, such as cell-cell and cell-matrix interactions, do not form. In contrast, 3D culture systems offer the advantage of a more tissue-like complexity and heterogeneity, cellular polarity and interactions. Volume and dead cells inside the spheroids, highlighted in green in the image on the right, are monitored over time through live-imaging.
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Tumor spheroids, obtained from lung cancer cells, treated with 3 concentrations of cytotoxic compound. Green fluorescence indicates cell death.
Read Our Method on Pubmed - Contract Research Services
Obinu and colleagues, supported by Kitos Biotech as contract service, evaluated the anticancer activity of their Poly (ethyl 2-cyanoacrylate) nanoparticles on our 3D tumor spheroids models of hepatocellular carcinoma and kidney adenocarcinoma cells. Their work was published on Colloids and Surfaces B: Biointerfaces.

​Link:
Obinu et al. Poly (ethyl 2-cyanoacrylate) nanoparticles (PECA-NP) as possible agents in tumor treatment. Colloids and Surfaces B: Biointerfaces (2019) 177: 520-528. Pubmed ID: 30822627

READ MORE ABOUT 3D MODELS >>                                          

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Kitos Biotech is a CRO that supports academics and companies in drug discovery of anticancer products.
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