Autophagic Flux Measurement
To monitor autophagic flux following drug treatment.
Features of the assay
- Quantitative autophagy detection by fluorescence microscopy.
Live cell imaging-based profiling of autophagy in HeLa cells. Cells were untreated or treated with Rapamycin and Chloroquine for 18 hours. Mean green fluorescence intensity for each cell, proportional to the amount of vescicles produced during autophagy, is quantified by digital imaging. Results are presented as boxplot of approximatively 1500 cells for each condition.