Kinetics of Cytotoxicity and ProliferationTo determine kinetics of compound potency and efficacy based on live-cell imaging.
Features of the assay
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Growth of A549 cells treated with 11 concentrations of Etoposide.
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Detailed description
Numerous methods that rely on proportionality between cell number and a cell's functional activity or a cellular marker, as MTT assay, are currently used to estimate cell number in cell viability assays. If a test compound affects the same functional activity or marker levels used to estimate cell number, the outcome of these methodologies will not be representative exclusively of cell number but also of the changes in that biomarker. This limitation is of extreme relevance for compounds which affect cell cycle progression or metabolic activity, as many anticancer agents. Furthermore, these methodologies do not discriminate reduced proliferation from increased cell death. Finally, they do not allow to monitor cell number more then one time in each well, which prevents to check the variability in the amount of cells in each sample immediately before compound treatment. To overcome these limitations, we validated a cytotoxicity and proliferation assay that directly count living cells and dead cells, in red and in green on the right image, enabling to monitor cell number over time with multiple readings. This methodology was in-house validated for high-throughput screenings. Contact us for further details.
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