Kinetics of Cytotoxicity and ProliferationTo determine kinetics of compound potency and efficacy based on live-cell imaging.
Features of the assay
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Direct Monitoring of Cell Number
Classic viability assays rely on the proportionality between cell number and a cell's functional activity or a cellular marker, as MTT assay. If a treatment affects the same functional activity or marker levels used to estimate cell number, the outcome of the assay will be representative of the changes in that biomarker, not of cell number. For instance, this limitation is of relevance for compounds which affect metabolic activity, as many anticancer agents. In contrast, our assay let to directly count exact number of cells and its outcome will be truly representative of cell viability.