Multi Drug Resistance Assay
To assess activity of ABC transporters following drug treatment.
Features of the assay
- Quantitative evaluation of ABC transporters by fluorescence microscopy.
Cells were treated with a mix of inhibitors of ABC transporters. Nuclei were highlighted in blue and multi-drug resistance inhibition is highlighted in green. Left image: untreated cells. Center image: treated cells. The mean green fluorescence intensity in each cell, proportional to inhibition of ABC transporters, was quantified by digital imaging. 3500 cells were quantified for each condition and Mann-Whitney U test was performed to assess statistical differences.